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Effect of C31 on VP30 phosphorylation. A, VP30-derived peptides identified by mass spectrometry. B, Label-free quantitative analysis of the high-resolution mass spectrometry spectra produced by Orbitrap MS scans for VP30 by SIEVE 2.1 software. Mean intensities (±SD) of the indicated VP30 peptides are shown. C, Quantification of nonphosphorylated KTCGSVEQQLNITAPK and phosphorylated KTCGSVEQQLNITAPKDSR peptides derived from the data in panel B. *P < .05.
