Table 1.

Binding of 1E7-03 Analogs to Protein Phosphatase 1 (PP1), Using Surface Plasmon Resonance

Compound Identifier	PP1 Wild Type	PP1 Triple Mutanta	PP1 Quadruple Mutantb
	KD (M)	KD (M)	KD (M)
1E7-03	6.16 × 10 -6	9.757 × 10 -6	9.042 × 10 -6
C17	9.6 × 10-7	No data	No data
C25	6.702 × 10-6	5.667 × 10-6	9.975 × 10-6
C31	1.88 × 10 -6	No data	8.01 × 10 -6
C33	1.412 × 10-5	1.300 × 10-5	1.006× 10-5
C34	2.55 × 10-5	2.533 × 10-5	6.671 × 10-5
C42	18.8 × 10-6	No data	No data
C44	5.26 × 10-6	No data	15.12 × 10-6
C46	3.245 × 10-4	2.756 × 10-4	3.676 × 10-4

The equilibrium dissociation constant (K_D) was calculated on the basis of a 1:1 binding model.

^aContains the mutations Y70W, L73Y, and A299E.

^bContains the mutations Y70W, L73Y, G274E, and A299E.