Figure 2.

Histopathology of peripheral myelinated nerves and ganglia in Marburg virus (MARV) infection. A, In a peripheral medium myelinated nerve, focal hemorrhage and histiocytic inflammation with necrotic cell debris dissecting between axons was present. B, Within the submandibular (Langley’s) parasympathetic autonomic ganglion, macrophages infiltrate and expand between ganglion cells, with necrotic cellular debris. There are rare viral intracytoplasmic inclusion bodies (arrows) (hematoxylin-eosin staining). C, Immunostains for MARV matrix protein label infiltrating macrophages, supporting Schwann cells, and neurofibroblasts (3,3′-diaminobenzidine [DAB] chromagen and hematoxylin counterstain). D, Infiltrating macrophages, supporting Schwann cells and neurofibroblasts express MARV glycoprotein in the ganglion. E, F, In situ hybridization for MARV genome labels infiltrating macrophages, supporting Schwann cells, and neurofibroblasts in the nerve (E) and ganglion (F) (red substrate chromagen and hematoxylin counterstain).