Figure 3.

Marburg virus (MARV) localization in the ovaries and oviducts. A, B, Cells of the ovarian theca interna surrounding secondary and tertiary follicles are diffusely positive for MARV matrix protein (VP40) and genomic RNA, respectively, with multifocal labeling of granulosa cells and ovarian stromal cells (3,3′-diaminobenzidine [DAB] and red substrate chromagen, respectively, and hematoxylin counterstain). C, D, Surface epithelium and subjacent stromal fibroblasts of the endosalpinx of the oviduct express MARV VP40 and contain viral genome. E, F, Within the myosalpinx of the oviduct, clusters of smooth muscle cells are positive for MARV VP40 antigen and genomic RNA.