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. 2018 Jul 19;218(Suppl 5):S423–S433. doi: 10.1093/infdis/jiy367

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Published by Oxford University Press for the Infectious Diseases Society of America 2018.

This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 4. — Marburg virus (MARV) localization in the uterus and testis. A, The superficial endometrial stroma of the uterus is diffusely positive for MARV matrix protein antigen (3,3′-diaminobenzidine [DAB] chromagen and hematoxylin counterstain). B, Strong and diffuse hybridization of viral genome in the endometrial stroma is present with rare labeling of endometrial gland epithelial cells (red substrate chromagen and hematoxylin counterstain). C, Ultrastructural appearance shown by transmission electron microscopy (TEM). Intracytoplasmic viral nucleocapsids are present in an endometrial stromal cell, with mature virus particles in the interstitium. Inset shows higher magnification of nucleocapsids. D, Testis. The interstitium between seminiferous tubules contains fibroblasts, Leydig cells, and blood vessels, labels for viral glycoprotein. E, Scattered labeling of testicular interstitial cells by antigenome in situ hybridization. F, TEM shows intracytoplasmic viral nucleocapsids within an interstitial fibroblast in the testis. Inset shows higher magnification of nucleocapsids.