Fig. 6. Absolute quantification of hsa-miR-132 and hsa-miR-146a in in vitro cultured cells (LPS stimulation) using amplified TG-FRET (blue) and RT-qPCR (red). miR-132 (left) and miR-146a (right) concentrations (in total-RNA extracts) of THP-1 cells after 0 h and 24 h of stimulation with LPS (1 μg ml–1). Note: value of miR-146a for 0 h stimulation measured with RT-qPCR is 0.9 and therefore not visible within the concentration scale. More information about the samples can be found in ESI Table S1.† All concentrations are given in copy number per ng of total RNA. Absolute (molar) concentrations are shown in ESI Fig. S3.† .
