Table 2.
Total and Relative Area Numbers Generated during the Validation Process Using Both Protocol Versions, Including the Average Difference of Each Method from the Manual Method.
| Total and Relative Areas Measured during Validation |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Total Area (microV*second) |
Relative Area (%) |
|||||||||
| QC |
VV |
QC |
VV |
|||||||
| N-Glycan | Andrew 1 (N = 52) | Andrew 2 (N = 40) | Manual (N = 23) | Andrew 1 (N = 48) | Manual (N = 6) | Andrew 1 (N = 52) | Andrew 2 (N = 40) | Manual (N = 23) | Andrew 1 (N = 48) | Manual (N = 6) |
| A2 | 159,358.77 | 150,642.29 | 231,777.00 | 181,610.26 | 243,364.58 | 1.12 | 1.26 | 1.58 | 1.67 | 1.71 |
| FA2 | 6,349,848.92 | 5,590,096.71 | 6,529,769.00 | 4,846,368.22 | 6,486,709.83 | 44.60 | 44.65 | 44.62 | 44.80 | 45.68 |
| FA2G1a | 2,921,047.82 | 2,607,333.65 | 2,998,212.28 | 2,231,314.23 | 2,878,339.67 | 20.49 | 20.66 | 20.49 | 20.64 | 20.27 |
| FA2G1b | 3,340,285.09 | 2,964,714.69 | 3,387,556.09 | 2,455,016.26 | 3,310,405.25 | 23.42 | 23.43 | 23.15 | 22.70 | 23.31 |
| FA2G2 | 1,293,861.37 | 1,149,093.61 | 1,339,268.13 | 996,007.11 | 1,202,646.08 | 9.06 | 9.06 | 9.15 | 9.21 | 8.47 |
| FA2G2Sg1/FA2G2Ga2 | 188,310.49 | 123,978.16 | 147,138.54 | 105,859.73 | 78,596.92 | 1.32 | 0.95 | 1.01 | 0.98 | 0.55 |
| Average difference from manual | 11.6% | 17.5% | 25.1% | 10.5% | 4.9% | 15.7% | ||||