Table 4.

Statistical Analysis of the Validation Data with a 95% Confidence Interval Using Both VV and QC Methods.¹⁵

t-Test Statistical Analysis Using a 95% Confidence Interval
QC Method (Pooled N = 117)		VV Method (Pooled N = 54)
t-Value Target	t-Value Achieved	t-Value Target	t-Value Achieved
1.96	1.84	2.01	6.91

t values were calculated using total glycan recovery. The total areas for all six monitored glycans were summed for each sample. The total recoveries were then averaged over all samples for the given method (automated QC, manual QC, automated VV, and manual VV).