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. 2018 Nov 14;38(11):469–479. doi: 10.1089/jir.2018.0066

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© Sophie Jacobs et al. 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 3. — Mouse IFN-λ titration using Fawa-λ-luc reporter cells and ELISA. (A) Luciferase activity detected in Fawa-λ-luc cells treated for the indicated time with 700 pg/mL mouse IFN-λ3 (mIFN-λ3). (B) Quantification by ELISA of mouse IFN-λ2 (mIFN-λ2) and mIFN-λ3 in cell supernatants. Two fold serial dilutions (800–12,800-fold) were quantified in quintuplicate. (C, D) Dose–response of mIFN-λ2 and mIFN-λ3 supernatants was measured in triplicate Fawa-λ-luc cells and is representative of at least 3 independent experiments. Cells were treated for 6 h with 2-fold serial dilutions. Data points in the linear range of the assays were plotted, and linear regression analysis was performed. LOD is based on the mean of mock treated cell signal, plus 3 standard deviations. ELISA, enzyme-linked immunosorbent assay; LOD, limit of detection; RLU, relative light units; R², coefficient of regression.