Table 1.
Expression Plasmids Used in This Study
| Vectors | Protein expressed, particularity | Type (parental vector) |
|---|---|---|
| CRISPR-Cas9 nickase expressing vectors | ||
| pSJ37 | sgRNA 1-exon 6 mouse IFNAR2c | Plasmid (px461) |
| pSJ38 | sgRNA 2-exon 6 mouse IFNAR2c | Plasmid (px461) |
| IFN-α expression plasmids | ||
| pcDNA3-IFNαA | IFNαA-mouse | Plasmid (pcDNA3) |
| pPH23 | IFNα2-human | Plasmid (pcDNA3) |
| IFN-λ expression plasmids | ||
| pSJ1 | IFN-λ2-mouse (C57bl/6 genomic sequence) | Plasmid (pcDNA3) |
| pSJ7 | IFN-λ3-mouse (C57bl/6 genomic sequence) | Plasmid (pcDNA3) |
| pCS59 | IFN-λ3-mouse (pEF-2-mIFN-λ3 derivative) | Plasmid (pcDNA3) |
| Lentiviral constructs | ||
| pMK03 | Mx1-promoter-Firefly luciferase | Lentiviral (pCCL) |
| pSJ12 | IFNLR1 (IRES-mCherry) | Lentiviral (pTM945) |
| Empty vector | ||
| pTM945 | IRES-mCherry | Lentiviral (pCCL) |
CRISPR, clustered regularly interspaced short palindromic repeats; IRES, internal ribosome entry site.