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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Gastrointest Endosc. 2018 Jul 3;88(4):755–763.e5. doi: 10.1016/j.gie.2018.06.022

Figure 5: HA-Tag AKT and MYC-tag beta catenin protein were expressed in swine hepatocytes and bile duct.

Figure 5:

Swine liver tissue that underwent hydrodynamic injection with AKT and beta-catenin plasmids were harvested at day 60, and tissues were analyzed for the expression of plasmid AKT and beta catenin protein via fluorescence microscopy. A–C show the anti HA-tag AKT protein inside hepatocytes. D–E demonstrates beta-catenin expressed in bile duct and hepatocytes. F illustrates that some hepatocytes express both AKT and beta-catenin. The figure demonstrate that hydrodynamic injection of plasmids can be stably integrated and expressed in swine hepatocytes and bile duct (amplification: 20X).