Table 2.
Growth medium for MenSCs
| Components | Reference | |||||
|---|---|---|---|---|---|---|
| Base | FBS (v/v) | Antibiotics | Other nutrients | Growth factors | ||
| Protocal 1 | DMEM | 20% | 1% penicillin and streptomycin | 1% glutamine | [25] | |
| Protocal 2 | DMEM/F12 | 10% | 100 U/mL penicillin and 100 mg/mL streptomycin | [20, 26] | ||
| Protocal 3 | Basic MSU-1 | 5% | 2 mM N-acetyl-l-cysteine, 0.2 mM l-ascorbic acid-2-phosphate | [74] | ||
| Protocal 4 | Minimal alpha GutaMAX | 10% | 10,000 U/mL penicillin, 10 mg/mL streptomycin and 25 μg/mL amphotericin in 0.9%NaCl | 20 mmol/L N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid pH 7.2–7.4 | 1.6 nmol/mL prednisolone sodium phosphate 80 μU/mL insulin 20 ng/mL FGF | [75] |