Table 1.

Primers and conditions used for RT-PCR to detect gene transcripts in hAESCs, HPCs, HLCs, and hPH

Genes	Sequence	TM(°C)	Size(bp)
GAPDH	F:5′-CCACCCATGGCAAATTCCATGGCA-3′	59	598
	R:5′-TCTAGACGGCAGGTCAGGTCCACC-3′
Nanog	F:5′-CAATGGTGTGACGCAGGGAT-3′	52	149
	R:5′-TGCACCAGGTCTGAGTGTTC-3′
OCT4	F:5′-ATCCCTGAACCTAGTGGGGA-3′	59	480
	R:5′-CACTCGGACCACATCCTTCT-3′
E-cadherin	F:5′-GGAAAATGCCCAGCCCGAC-3′	60	210
	R:5′-ACGTTATATGCCCGCACGAT-3′
CK7	F:5′-TTTGTGGTGCTGAAGAAGGATG-3′	57	683
	R:5′-CCACCAGTGGAATTCATCACA-3′
CD105	F:5′-TCCTCCCAAGGACACTTGTA-3′	57	244
	R:5′-CGCCTCATTGCTGATCATAC-3′
CD73	F:5′-CTCCTCTCAATCATGCCGCT-3′	55	174
	R:5′-TGGATTCCATTGTTGCGTTCA-3′
CD90	F:5′-GAGGGAGGAAGAGCAGACCT-3′	57	896
	R:5′-CCTGGATCGGGTTATGATGGG-3′
HLA-DR	F:5′-CAAGAGCTCCAACTATACTCCGAT-3′	63	273
	R:5′-ACCCTGCAGTCGTAAACGTCC-3′
HLA-ABC	F:5′-GTATTTCTTCACATCCGTGTCCCG-3′	65	394
	R:5′-GTCCGCCGCGGTCCAAGAGCGCAG-3′
ALB	F:5′-GGTGAGACCAGAGGTTGATGTG-3′	61	695
	R:5′-GCAGCAGCACGACAGAGTAATC-3′
AAT	F:5′-TGAGTTCGCCTTCAGCCTATACC-3′	61	530
	R:5′-AGTCCTCTTCCTCGGTGTCCTT-3′
CYP3A4	F:5′-ATGGTCAACAGCCTGTGCT-3′	57	317
	R:5′-CATGCTGTAGGCCCCAAAGA-3′
CK19	F:5′-GCTTTGTGTCCTCGTCCTCC-3′	57	614
	R:5′-TTGGCTTCGCATGTCACTCA-3′
CK18	F:5′-ATGAGCTTCACCACTCGCTC-3′	57	688
	R:5′-TGGCAATCTGGGCTTGTAGG-3′
CK7	F:5′-CCCAGACATCTTTGAGGCCC-3′	57	973
	R:5′-TTCACGGCTCCCACTCCATC-3′
AFP	F:5′-AGTTTAGCTGACCTGGCTACC-3′	55	275
	F:5′-GGAGTGGGCTTTTTGTGTGC-3′

F forward primer, R reverse primer