FIGURE 3:
Rnd3 knockdown enhances the fibrotic phenotype in normal lung fibroblasts. MRC5 cells were transfected with control, Rnd3 #1, or Rnd3 #2 siRNA for 72 h. (A) Cell lysates were analyzed by Western blotting for Rnd3 and Erk2 (loading control). (B) Cell lysates were analyzed for p190 activity through a GST-RhoAQ63L pull-down assay. (C) Quantification of p190 activity from three independent assays. (D) Cell lysates were analyzed for RhoA activity through a GST-RBD pull-down assay. (E) Quantification of RhoA activity from three independent assays. (F) Total cell lysates were analyzed by Western blot for expression of FN, collagen I, SMA, and Erk2 (loading control). *p < 0.05 vs control as determined by a t test.