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. 2018 Sep 1;29(18):2165–2175. doi: 10.1091/mbc.E17-11-0642

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© 2018 Monaghan-Benson et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 8: — Rnd3 overexpression abolishes IPF phenotype in response to substrate stiffness. LL29 cells were transfected with Rnd3 (where indicated). Cells were then plated on substrates with stiffnesses of 2, 8, and 25 kPa. (A) Cells were lysed and levels of GST-Rnd3 and Erk2 (loading control) were evaluated by Western blot. (B) Cells were then analyzed for p190 activity using a GST-RhoA^Q63L pull-down assay. (C) Cell lysates were then analyzed for the activation of RhoA measured through a GST-RBD pull-down assay. (D) LL29 cells were lysed, and lysates evaluated by Western blot for FN, collagen I, SMA, and Erk2 levels.