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. 2018 Sep 1;29(18):2190–2200. doi: 10.1091/mbc.E17-12-0743

FIGURE 7:

FIGURE 7:

Vam3-dependent vacuole fusion is a requirement of ETA mediated autophagy rescue in snx4Δpsd1Δ cells. (A) Maximum projection micrographs of WT and vam3Δ cells expressing GFP-Atg8 before and after addition of ethanolamine (50 mM) are shown. The scale bars indicate 5 μm. (B) Maximum projection micrographs of snx4Δpsd1Δ and snx4Δpsd1Δvam3Δ cells expressing GFP-Atg8 treated similarly are shown. (C) The number of GFP-Atg8 puncta per cell was quantified at the indicated time points after ETA addition in each of the four strains. A minimum of 19 cells were analyzed in each condition and plotted as individual dots as indicated and the mean and SD are indicated.