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. 2018 Sep 15;29(19):2259–2279. doi: 10.1091/mbc.E18-04-0225

FIGURE 6:

FIGURE 6:

Deletion of ync13 affects localization and dynamics of TRAPP-II complex and Rab11 GTPase Ypt3 at the division site during cytokinesis. (A) Trs120 accumulates at the center of division site in ync13∆ cells. The middle focal planes are shown with the sum projection (GFP channel) of a 2-min continuous movie and DIC. (B) Syb1 accumulates at the center of division plane in ync13∆ cells. (C–F) ync13∆ affects Ypt3 distribution (C, D), dynamics (E), and tethering/docking time (F). (C) The sum projection in GFP channel is from a 2-min continuous movie. (D) Area scans of Ypt3 distribution in the sum projection during septum maturation. (E) Kymographs of the division site (vertical) of the numbered cells in C during ring constriction (cells 1 and 2) or septum maturation (cells 3 and 4). The arrows mark stable Ypt3 at the leading edge (cell 2) or the center of septum (cell 4). (F) Ypt3 tethering/docking time at the center of division plane is lengthened in ync13∆ cells. The division plane was divided to the center half and rim half. (G) The final deposition site of Ypt3 along the division site during septum maturation. Bars, 5 µm.