TABLE 1:
Genetic interactions between mutations in ync13 and others affecting cytokinesis and membrane trafficking.a
| Mutations | 25°C | 30°C | 32°C | 36°C |
|---|---|---|---|---|
| ync13-4 | +++b | +++ | ++c | +d |
| Contractile ring | ||||
| myo2-E1 | +++ | ++ | + | + |
| myo2-E1 ync13-4 | ++ | + | –e | – |
| cdc15-140 | +++ | + | – | – |
| cdc15-140 ync13-4 | +++ | + | – | – |
| imp2∆ | + | /f | / | / |
| imp2∆ ync13-4 | – | / | / | / |
| fic1∆ | +++ | +++ | ++ | ++ |
| fic1∆ ync13-4 | +++ | +++ | ++ | + |
| pxl1∆ | +++ | +++ | +++ | ++ |
| pxl1∆ ync13-4 | +++ | ++ | + | + |
| Glucan synthases and glucanases | ||||
| bgs1-191 | +++ | +++ | ++ | – |
| bgs1-191 ync13-4 | ++ | ++ | – | – |
| cwg1-2 | +++ | +++ | ++ | + |
| cwg1-2 ync13-4 | +++ | ++ | + | – |
| mok1-664 | +++ | +++ | + | – |
| mok1-664 ync13-4 | +++ | +++ | – | – |
| eng1∆ | +++ | +++ | +++ | +++ |
| eng1∆ ync13-4 | +++ | +++ | +++ | ++ |
| agn1∆ | +++ | +++ | +++ | +++ |
| agn1∆ ync13-4 | +++ | +++ | ++ | + |
| Rho GTPase-dependent CIP | ||||
| rho1-596 | +++ | / | / | / |
| rho1-596 ync13-4 | – | / | / | / |
| rho2∆ | +++ | +++ | +++ | +++ |
| rho2∆ ync13-4 | +++ | +++ | ++ | + |
| art1∆ | ++ | / | / | / |
| art1∆ ync13-4 | – | / | / | / |
| rga7∆ | ++ | / | / | / |
| rga7∆ ync13-4 | – | / | / | / |
| pck1∆ | +++ | +++ | +++ | ++ |
| pck1∆ ync13-4 | + | – | – | – |
| pck2∆ | +++ | +++ | ++ | ++ |
| pck2∆ ync13-4 | +++ | +++ | + | + |
| Exocytosis | ||||
| sec8-1 | +++ | ++ | ++ | – |
| ync13-19 | +++ | + | – | – |
| sec8-1 ync13-4 | ++ | + | – | – |
| sec8-1 ync13-19 | ++ | + | – | – |
| exo70∆ | +++ | +++ | +++ | ++ |
| exo70∆ ync13-4 | +++ | +++ | ++ | – |
| sec3-913 | +++ | +++ | ++ | + |
| sec3-913 ync13-4 | +++ | +++ | + | – |
| sec3-916 | +++ | +++ | ++ | – |
| sec3-916 ync13-4 | + | – | – | – |
| myo52∆ | +++ | ++ | ++ | + |
| myo52∆ ync13-4 | +++ | ++ | + | + |
| cdc42-1625 | +++ | / | / | / |
| cdc42-1625 ync13-4 | – | / | / | / |
| rho3∆ | +++ | +++ | +++ | +++ |
| rho3∆ ync13-4 | +++ | +++ | + | + |
| for3∆ | ++ | ++ | ++ | ++ |
| for3∆ ync13-19 | ++ | + | – | – |
| trs120-ts1 | +++ | +++ | +++ | – |
| trs120-ts1 ync13-4 | +++ | +++ | ++ | – |
| Endocytosis | ||||
| end4∆ | + | – | – | – |
| end4∆ ync13-4 | + | – | – | – |
| pan1∆ACV | +++ | +++ | +++ | +++ |
| pan1∆ACV ync13-4 | +++ | +++ | ++ | + |
| fim1∆ | +++ | +++ | +++ | ++ |
| fim1∆ ync13-4 | +++ | +++ | ++ | + |
| arp2-1 | +++ | +++ | ++ | + |
| arp2-1 ync13-4 | +++ | +++ | ++ | + |
| acp2∆ | +++ | +++ | +++ | +++ |
| acp2∆ ync13-4 | +++ | +++ | ++ | + |
| wsp1∆ | +++ | +++ | +++ | +++ |
| wsp1∆ ync13-4 | +++ | +++ | ++ | ++ |
| Other mutants | ||||
| its3-1 | +++ | ++ | + | – |
| its3-1 ync13-4 | +++ | ++ | + | – |
| rho4∆ | +++ | +++ | +++ | +++ |
| rho4∆ ync13-4 | +++ | +++ | +++ | ++ |
| Grown on EMM5S | ||||
| ync13∆ | ++ | ++ | ++ | ++ |
| lad1-1(rgf3) | +++ | +++ | ++ | ++ |
| lad1-1(rgf3) ync13∆ | ++ | + | – | – |
| Grown on YE5S | ||||
| ync13∆ | – | / | / | / |
| eng1∆ | +++ | / | / | / |
| eng1∆ ync13∆ | ++ | / | / | / |
| agn1∆ | +++ | / | / | / |
| agn1∆ ync13∆ | – | / | / | / |
aGrowth and color of colonies on YE5S + phloxin B plates except where noted at various temperatures.
b+++, similar to wt.
c++, some cell lysis or cytokinesis defects.
d+, massive cell lysis, severe cytokinesis defects with reduced growth.
e–, inviable.
f/, not tested.