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. 2018 Nov 1;29(22):2751–2765. doi: 10.1091/mbc.E18-03-0158

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© 2018 Varlakhanova et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — Par32 electrophoretic mobility shifts are defined by nutrient availability. (A) Mobility shifts of Par32-3xHA under various nutrient conditions in W303A and Δnpr1 cells. W303A or Δnpr1 cells expressing the indicated 3xHA-tagged construct were grown under the indicated conditions for 3 h prior to cell lysis and blotting with an anti-HA antibody. Pgk1 was used as a loading control. (B) As in A but using nitrogen starvation for the indicated times. (C) As in B but using glucose starvation for the indicated times. (D) W303A or Δnpr1 cells expressing various Par32-3xHA constructs grown in the indicated conditions for 3 h. Pgk1 was used as a loading control.