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. 2018 Nov 1;29(22):2766–2783. doi: 10.1091/mbc.E18-07-0434

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© 2018 Di Cara et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 7: — Dysfunctional peroxisomes in gut epithelial cells lead to dysbiosis of the gut microbiota and compromised host-pathogen interaction. Quantification of total bacteria (A) and bacillus-specific bacteria (B) assayed by qPCR of the 16S rRNA gene in dissected intestines from control and Mex >Pex5-i flies. Values reported represent the average of four independent experiments. Statistical significance was determined using Student’s t test; ****p < 0.0001. (C) Survival of adult control and Mex >Pex5-i flies fed 5% sucrose or under chronic oral infection with Ecc15. Values reported represent the averages of three independent experiments ± SD. Statistical significance was determined using the log-rank test (Mantel-Cox); ****p < 0.0001; **p < 0.01; ns = not significant. (D) Adult flies of the indicated genotypes were infected with Ecc15. Bacteria were isolated from flies and plated onto solid nutrient medium, and the numbers of CFUs were counted 1, 2, and 3 d after infection. Note the significant increases in bacterial load in infected Mex >Pex5-i flies. Data report the mean ± SD. Statistical significance was determined using two-way ANOVA; ****p < 0.0001. (E) Relative amounts of endogenous H₂O₂ in midguts of adult control flies, Mex >Pex5-i flies and Mex >Cat-i flies before infection (0 h) and at 2, 5, and 24 h postinfection with Ecc15. n = 15 for each genotype in a single experiment. Values reported represent the average of three independent experiments ± SD for each genotype at each time point. Statistical significance was determined using two-way ANOVA; ***p < 0.001; **p < 0.01; *p < 0.05. (F) Quantification of the fold change in Duox expression in adult guts from control and Mex >Pex5-i flies after infection with Ecc15 relative to guts from uninfected flies of the corresponding genotype. Values reported are the averages of three independent experiments ± SD. Statistical significance was determined using one-way ANOVA; ****p < 0.0001; *p < 0.05. (G) Quantification of the fold change in Dpt expression in adult midguts from control and Mex >Pex5-i flies after infection with Ecc15 relative to midguts from uninfected flies of the corresponding genotype. Values reported are the averages of three independent experiments ± SD. Statistical significance was determined using one-way ANOVA; ****p < 0.0001.