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. 2018 Nov 1;29(22):2632–2643. doi: 10.1091/mbc.E18-01-0026

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© 2018 Uetake and Sluder. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 6: — Transient post–mitotic activation of p38 in daughter cells born of mothers that spent 2–6 h in prometaphase. Western blots for activated p38, top row (phospho Thr180/Tyr182), and total p38, bottom row. First lane: mitotic shake-off cells from control culture. 0 min–5 h lanes: asynchronous cultures were treated with nocodazole for 4 h; mitotic cells were shaken off and cultured another 2 h in nocodazole before drug removal. Populations were prepared for blotting at the indicated times after nocodazole removal. 0 min lane: prometaphase cells collected just after drug removal. 40 min lane: late anaphase/telophase cells harvested 40 min after nocodazole removal. 1.5 h lane: G1 cells collected 1.5 h after nocodazole removal. 5 h lane: G1 cells harvested 5 h after nocodazole removal (from a different blot). The blots shown are representative of five experiments that showed identical results.