TABLE 2:
Yeast strains used in this study.
| Strain | Genotype | Reference or source |
|---|---|---|
| BY4741 | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 | Research Genetics |
| JTY4470 | BY4741 ste2Δ::KanMX4 | Research Genetics |
| yAEA201 | BY4741 STE2prom::STE2-FLAG-(His)6::URA3 | This study |
| yAEA152 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 [FAP-Ste2] | This study |
| yAEA265 | BY4741 STE2prom::STE2-FLAG-(His)6::URA3 bar1Δ::KanMX | This study |
| yAEA261 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 bar1Δ::KanMX4 | This study |
| DK102 | BY4741 ste2Δ::HIS3 bar1Δ | D. Kaim, this lab |
| yAEA361 | BY4741 STE2prom::STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX | This study |
| yAEA363 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 yps1Δ::KanMX | This study |
| yAEA365 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 mkc7Δ::KanMX | This study |
| yAEA359 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX | This study |
| JTY6757 | BY4741 STE2prom::STE2-EGFP::HphNT1 | Alvaro et al., 2014 |
| YEL014 | BY4741 STE2prom::STE2-mCherry::CaURA3 | E. Sartorel, this lab |
| YDB103 | BY4741 sst2Δ::KanMX ste2Δ | Ballon et al., 2006 |
| yAEA260 | BY4741 STE2prom::STE2-FLAG-(His)6::URA3 sst2::HphNT1 | This study |
| yAEA372 | BY4741 STE2prom::STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX sst2::HphNT1 | This study |
| yAEA373 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX sst2Δ::HphNT1 | This study |
| yAEA257 | BY4741 STE2prom::STE2-EGFP::HphNT1 sst2Δ::KanMX | This study |
| yAEA258 | BY4741 STE2prom::STE2-mCherry::CaURA3 sst2Δ::HphNT1 | This study |
| yAEA382 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX glo3Δ::HphNT1 VPH1-EGFP::HIS3 | This study |
| yAEA379 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX ldb19Δ::NatMX rog3Δ::KanMX rod1Δ::KanMX | This study |
| yAEA380 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX VPH1-EGFP::HIS3 | This study |
| yAEA381 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX ldb19Δ::NatMX rog3Δ::KanMX rod1Δ::KanMX VPH1-EGFP::HIS3 | This study |
| yAEA383 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX ldb19Δ::NatMX VPH1-EGFP::HIS3 | This study |
| yAEA384 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX rod1Δ::KanMX VPH1-EGFP::HIS3 | This study |
| yAEA385 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX rog3Δ::KanMX VPH1-EGFP::HIS3 | This study |
| yAEA388 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::URA3 yps1Δ::KanMX mkc7Δ::KanMX rod1Δ::KanMX rog3Δ::KanMX VPH1-EGFP::HIS3 | This study |
| yAEA389 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2-FLAG-(His)6::HphNT1 yps1Δ::KanMX mkc7Δ::KanMX ldb19Δ::NatMX rog3Δ::KanMX rod1Δ::KanMX VPH1-EGFP::HIS3 | This study |
| yAEA397 | BY4741 STE2prom::MFα1(1-83)-Igκ-FAPα2-STE2(7K-to-R)-FLAG-(His)6::URA3 yps1::KanMX mkc7::KanMX [FAP-Ste2(7K-to-R)] | This study |