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U2OS cells were transfected with GFP-Cep215 for 18 h. On the day of imaging, the cells were incubated in freshly prepared phenol red-free DMEM along with Alexa Fluor568-conjugated transferrin for 15 min. The medium was then replaced with fresh phenol red-free DMEM and cells were transferred to a heated chamber with 5% CO2 for live cell imaging. Images were taken every 2 s for 10 min. The processed video shows images at 15 frames per second.
