Video S2.

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Rap1^N17 border cells are less organized and fail to complete their migration. Example of a live stage 9 Rap1^N17 (c306-GAL4, tsGAL80/+; UAS-Rap1^N17/slbo-LifeAct-GFP) egg chamber in which the rear border cell remains attached to an anterior follicle cell. Border cells extend longer side protrusions during the first half of the Video (until ~1:18; time in h:min), after which border cells become round. By the end of the Video, border cells in the cluster are less organized than in control. Border cell migration is slower than in the control and the cluster does not reach the oocyte by the correct stage. Frames were acquired every 3 min with a 20x objective. Anterior is to the left.