FIGURE 2:

CSC can stimulate membrane-deformation activity of the SNX-BAR protein complex. (A) Control of SNX-BAR protein on membranes. Purified GFP-tagged SNX-BAR complex (30 nM) was added to GUVs and analyzed as in Figure 1C. (B) CSC has no affinity for membranes. Limiting amounts of CSC (30 nM) were incubated with the GUVs in the absence of the SNX-BAR complex. (C, D) Titration of CSC to SNX-BAR–bearing membranes. The indicated amounts of RFP-tagged CSC (Vps29-RFP) was added to membranes together with the previously (in A) determined amount of GFP-tagged SNX-BAR complex. GUV membranes were stained as in Figure 1B. (E) Quantification of the results. Tubulation was quantified as described under Materials and Methods. Data are represented as mean ± SD. Scale bar, 5 µm. Note that under the selected conditions, 30 nM of SNX-BAR had very little effect on membrane tubulation here.