FIGURE 5:

CSC can trigger Snx3-driven membrane deformation. (A–D) Limiting amounts of Snx3-mGFP (5 nM) were added alone (A) or together with increasing amounts of CSC (5–30 nM, B–D) to GUVs as in Figure 3, incubated for 15 min, and then examined by fluorescence microscopy. (E) Quantification of vesiculation in the presence of SNX3-mGFP at the indicated amounts of CSC. The quantification of Snx3-GFP induced deformation is the same as in Figure 4G and is shown here for comparison. Data are represented as mean ± SD. Scale bar, 5 µm.