Table 1.
Identification of 2-DE spots from parental and mutant lines by MS/MS
| Cultivar | Spot # | Predominant Protein | Accession Number | # Exclusive Unique Peptides | # Spectra | % Coverage |
|---|---|---|---|---|---|---|
| Keumkang | 1 | omega-5 gliadin | CAR82267 | 16 | 47 | 29 |
| Keumkang | 2 | omega-5 gliadin | BAE20328 | 16 | 48 | 25 |
| Keumkang | 3 | omega-5 gliadin | BAE20328 | 28 | 59 | 38 |
| Keumkang | 4 | omega-5 gliadin | BAE20328 | 6 | 12 | 19 |
| Keumkang | 5 | omega-5 gliadin | BAE20328 | 15 | 41 | 40 |
| Keumkang | 6 | LMW-GS (i-type)a | AAS10189 | 59 | 180 | 81 |
| Keumkang | 7 | LMW-GS (s-type)b | ACA63869 | 67 | 191 | 73 |
| Keumkang | 8 | LMW-GS (s-type)b | ACA63869 | 64 | 211 | 72 |
| Keumkang | 9 | LMW-GS (s-type)b | ACA63869 | 90 | 305 | 76 |
| Keumkang | 10 | LMW-GS (s-type)b | ACA63869 | 130 | 443 | 88 |
| Keumkang | 11 | LMW-GS (s-type)b | ACA63865 | 90 | 313 | 78 |
| Keumkang | 12 | triticin | ACB41345 | 30 | 74 | 29 |
| Keumkang | 13 | gamma gliadin | Bu-5d | 53 | 177 | 60 |
| Keumkang | 14 | gamma gliadin | Bu-5d | 47 | 164 | 61 |
| Keumkang | 15 | gamma gliadin | AGJ50340 | 46 | 164 | 62 |
| Keumkang | 16 | LMW-GS (m-type)c | ABC84361 | 66 | 179 | 74 |
| Keumkang | 17 | LMW-GS (m-type)c | ACA63871 | 70 | 357 | 84 |
| Olgeuru | 1 | omega-5 | AIU64835 | 7 | 26 | 48 |
| Olgeuru | 2 | omega-5 | AII26682 | 6 | 15 | 17 |
| Olgeuru | 3 | omega-5 | AII26682 | 4 | 15 | 16 |
| Olgeuru | 4 | omega-5 | CAR82266 | 6 | 13 | 25 |
| Olgeuru | 5 | omega-5 | BAE20328 | 36 | 75 | 45 |
| Olgeuru | 6 | omega-5 | AII26682 | 9 | 25 | 34 |
| Olgeuru | 7 | LMW-GS (i-type)a | BAB78764 | 60 | 175 | 77 |
| Olgeuru | 8 | LMW-GS (s-type)b | ACA63864 | 40 | 110 | 54 |
| Olgeuru | 9 | LMW-GS (s-type)b | ACA63868 | 44 | 124 | 62 |
| Olgeuru | 10 | LMW-GS (s-type)b | ACA63864 | 92 | 310 | 73 |
| Olgeuru | 11 | LMW-GS (i-type)c | ACY08811 | 47 | 111 | 69 |
| Olgeuru | 12 | triticin | ACB41345 | 24 | 60 | 27 |
| Olgeuru | 13 | gamma gliadin | AGO17716 | 23 | 51 | 45 |
| Olgeuru | 14 | gamma gliadin | AGO17716 | 31 | 74 | 57 |
| Olgeuru | 15 | LMW-GS (m-type)c | ABC84361 | 60 | 164 | 74 |
| Olgeuru | 16 | LMW-GS (m-type)c | ACP27643 | 23 | 118 | 51 |
| DH20 | 1 | LMW-GS (i-type)a | AAS10189 | 104 | 310 | 83 |
| DH20 | 2 | LMW-GS (i-type)a | AAS10189 | 30 | 98 | 70 |
| DH20 | 3 | triticin | ACB41345 | 39 | 129 | 37 |
| DH20 | 4 | gamma gliadin | AGJ50340 | 49 | 161 | 58 |
| DH20 | 5 | LMW-GS (m-type)c | ABC84361 | 60 | 164 | 74 |
arefers to LMW-GS that begin with the N-terminal sequence ISQQQ
brefers to LMW-GS that begin with the N-terminal sequence SHIP
crefers to LMW-GS that begin with M
dbest match is to a sequence from cv. Butte 86 reported in [19]