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. 2018 Nov 15;29(23):2809–2820. doi: 10.1091/mbc.E18-09-0590

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© 2018 Hung et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 1: — Generation of TruHD-immortalized cell lines. (A) hTERT mRNA levels normalized to β-actin mRNA levels in RPE1 cells (positive control), primary cells, and TruHD cells. hTERT levels in primary cells were not detectable (ND). n = 5. Error bars represent SEM. *p = 0.0369 comparing TruHD-Q21Q18F, TruHD-Q43Q17M, and TruHD-Q50Q40F by one-way analysis of variance (ANOVA). (B) Telomeric repeat amplification product (TRAP) assay. Amplification products run on 10% TBE gel after telomere extension reaction, showing telomeric repeats >50 base pairs in increments of 6 base pairs. Template strand is 36 base pairs. Negative control contains no Taq polymerase or template strand. (C) Representative karyotypes of TruHD-Q21Q18F, TruHD-Q43Q17M, and TruHD-Q50Q40F cells. “mar” denotes marker chromosomes, “+” are additional chromosomes and “?add(4)(p14)” denotes additional patterns observed on chromosome 4 at band p14. Results from full karyotype shown in Table 2.