Figure 4:

Cells grown on a nonfermentative carbon source tolerate Msn2A6 expression. (A) W303 wild-type cells carrying plasmids pGAL1-10-MSN2A6 and pADGEV were grown to logarithmic phase in liquid synthetic media containing either glucose or ethanol/glycerol as a carbon source and subsequently exposed to a range of 17-ß-estradiol concentrations. Growth (OD_600nm) was recorded for 48 h. Ratios of treated vs. untreated growth are shown. Cells transformed with plasmids pGAL1-10-MSN2A6 and plasmids pRS316 (instead of pADGEV) served as negative controls. (B) W303 wild-type cells carrying plasmid pGAL1-10-MSN2A6 and pADGEV (control: pRS316) were grown in liquid synthetic media in the presence of ethanol/glycerol as sole carbon source. Msn2A6 expression was induced by 2 or 10 nM 17-ß-estradiol. Growth was recorded for 26 h and compared with that of negative controls (pRS316 instead of pADGEV). (C) Mutants with a predisposition for utilization of nonfermentative carbon sources (pfk1∆, pfk27∆, tps3∆) have reduced growth inhibition upon induction of Msn2A6 with 17-ß-estradiol.