Figure 5:

Oxygen consumption rates increase in Msn2A6-expressing cells. (A) W303 wild-type cells carrying plasmids pGAL1-10-MSN2A6 and pADGEV were grown in liquid synthetic media containing glucose. Black lines indicate addition of 17-ß-estradiol (100 nM) and 2% ethanol to the cultures. Growth (OD₆₀₀) was recorded every 2 h. (B) Molecular oxygen consumption [µM s^–1] of all cultures was determined from the linear decrease in oxygen concentration followed polarographically for 1 min using a Clark-type oxygen electrode at 30°C. (B) Oxygen consumption normalized to growth rate. (C) Oxygen consumption rate relative to cell density.