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. 2018 Nov 21;9:324. doi: 10.1186/s13287-018-1062-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Macroscopic and microscopic structure of the rat testis after SDS-based decellularization process. A lyophilized decellularized testis scaffold showed whitish translucent appearance (a). The transverse section of the decellularized testis showed intact ECM and tunica albuginea gross architecture. DNA quantification showed significant cell removal by decellularization procedure (N = 3) (b). Comparing of the H&E (c, d), Hoechst (e, f), staining of the naïve (c, e), and decellularized tissue (d, f) also confirmed the cell removal. Both staining showed that ECM was preserved with no residual nuclei or cells