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. 2018 Dec;26(12):999–1007. doi: 10.1016/j.tim.2018.06.004

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Outline of a Holistic Stable-Isotope Probing (SIP) Experiment. SIP technologies are integrated with multi-omics, microscopic and chemical imaging to provide a comprehensive picture of the microbiome dynamics and activities, and specific identities and physiological interactions of isotope-labelled microorganisms. Thick black arrow with clock indicates the timeline of an experiment. Thick and thin blue arrows indicate sampling time-points during the experiment for different analyses. IRMS, isotope ratio mass spectrometry. FISH, fluorescence in situ hybridization.