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. 2018 Nov 8;2018:6808934. doi: 10.1155/2018/6808934

Figure 1.

Figure 1

RIG-I is dispensable for survival and weight loss during influenza infection. (a) Wild-type (WT), but not RIG-I KO, mice express RIG-I in isolated type II alveolar epithelial cells (AEC). AEC II were infected with IAV PR8 at an MOI of 6 and incubated for an additional 24 h to stimulate RIG-I production. The cells were processed for immunohistochemistry for detection of RIG-I (red). Scale bars = 50 μm. (b) WT, but not RIG-I KO, mice infected with IAV express RIG-I in the lung. Immunohistochemical staining of RIG-I and IAV nucleoprotein (NP) in WT and RIG-I KO mice. Mice were intranasally infected with 300 pfu IAV PR8 or mock infected with PBS. Mouse lungs were processed for immunohistochemistry for detection of RIG-I protein (green) or IAV NP (red). The bar represents 100 μm. (c and d) RIG-I KO and littermate WT mice were intranasally inoculated with IAV at 1000 pfu/mouse. Mortality (c) and body weights (d) were monitored daily. Body weight data were normalized to each mouse's starting body weight. Data are expressed as mean ± standard deviation (n = 13 for RIG-I KO mice; n = 15 for WT mice).