Figure 7.

The absence of RIG-I or MAVS does not inhibit induction of interferon or generation of an inflammatory response during IAV infection. WT, RIG-I KO, and MAVS KO mice were mock-infected or infected with the IAV PR8 strain (300 PFU/mouse). At day 6 postinfection, lung tissue was collected, and total RNA was isolated and analyzed for RNA-Seq using 3′ inTAG next-generation sequencing (n = 6). (a) Venn diagram showing the distribution of shared differentially expressed genes during IAV infection. Heat map comparing the genes of interferon (b) and inflammatory responses (c). The log2 counts per million for the corresponding gene sets were averaged for each group and clustered as heat maps using the Euclidean distance and Ward clustering metric. The yellow/blue gradient indicates high/low gene expression, respectively.