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. 2018 Nov 8;2018:9364364. doi: 10.1155/2018/9364364

Figure 7.

Figure 7

Knockdown of Nrf2 attenuated antioxidant enzyme expression induced by TBE and GA in LPS-stimulated macrophages. (a) RAW 264 cells were transfected with 60 nM Nrf2 siRNA or NC siRNA for 48 h. The mRNA levels of Nfe2l2 were detected by real-time RT-PCR. Data represent mean ± SD, n = 3 (∗∗∗ p < 0.001 compared to NS group). (b) After transfection of 60 nM Nrf2 siRNA or NC siRNA for 48 h, RAW 264 cells were pretreated with 400 μg/mL TBE or 46 μg/mL GA for 1 h, followed by treatment with 100 ng/mL LPS for 4 h. The mRNA levels of Hmox1, Cat, Nqo1, and Gclm were detected by real-time RT-PCR. Data represent mean ± SD, n = 3 (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; NS, no significant difference).