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. Author manuscript; available in PMC: 2019 Nov 14.
Published in final edited form as: Cell Host Microbe. 2018 Nov 14;24(5):743–750.e5. doi: 10.1016/j.chom.2018.09.015

Figure 2. Sera from mice born to ZIKV-immune mothers neutralize ZIKV but not DENV2 infection.

Figure 2.

Serum was collected from 4- to 5-week-old LysMCre+Ifnar1fl/fl mice born to mothers previously infected with ZIKV strain SD001 (106 FFU via retro-orbital route) or naïve mothers. Serum of pups (open squares, n=10) from naïve mothers (n=2) were compared to pups (black circles, n=13) from ZIKV-immune mothers infected for 6-13 months (n=4). (A) anti-ZIKV IgG and (B) anti-DENV IgG were detected via ELISA. Neutralization capacity was assessed against (C) ZIKV SD001 and (D) DENV S221 via U937-DC-SIGN cells and a flow cytometry-based assay (Wen et al., 2017). Dotted line indicates limit of detection for ELISA and 50% or 0% neutralization line for the neutralization assay. Serum of pups (black circles, n=13) from ZIKV-immune mothers infected for 6-13 months (n=4) were compared to serum of pups (grey circles, n=10) from ZIKV-immune mothers infected for 2 months (n=3). (E) anti-ZIKV IgG and (F) anti-DENV IgG were detected via ELISA. Data are pooled from 3 independent experiments and are expressed as mean +/− standard error of mean.