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. Author manuscript; available in PMC: 2019 Nov 14.
Published in final edited form as: Cell Host Microbe. 2018 Nov 14;24(5):743–750.e5. doi: 10.1016/j.chom.2018.09.015

Figure 3. Passive transfer of IgG isolated from mice born to ZIKV-immune mothers into naïve mice increases viral burden upon DENV2 challenge.

Figure 3.

IgG was isolated from serum of 36 pups born to ZIKV-immune mothers infected for 6-12 months (n=8) or from 32 age-matched naïve mice from naïve LysMCre+Ifnar1fl/fl mothers (n=7). (A) 145 μg or (B) 14.5 μg of purified IgG isolated from pups born to ZIKV-immune mothers (ZIKV IgG) or naïve mothers (naïve IgG) was passively transferred into naïve 4- to 5-week old LysMCre+Ifnar1fl/fl recipient mice, followed by challenge of the passively transferred recipient mice with 2 × 105 FFU of DENV2 strain S221 via tail vein injection. Tissues were harvested 3 days p.i. and DENV2 RNA levels in the (A and B) serum, and spleen, and liver were quantified by qRT-PCR. n=6-7 ZIKV IgG recipient mice and n=6 naïve IgG recipient mice. Mann-Whitney test (** P < 0.01, * P < 0.05).