Figure 1. Incubation of P. tricornutum on plates containing different concentrations of blasticidin-S.
2.5 × 107 P. tricornutum cells were spread on plates (25% salinity of seawater) and containing different concentrations of blasticidin-S. At concentrations between 0 and two μg/ml of blasticidin-S (A–E) we observed a lawn of cells. At 2.5 μg/ml (F) and 3.0 μg/ml (G) we observed scattered growth, and above 3.5 μg/ml (H) no growth was observed. Magnified areas in the picture are indicated by an arrow.