Fig. 5. GSH depletion impairs iron-sensing in A. fumigatus. Strains were pre-incubated at 25 °C for 20 h in MM +Fe with 5 mM GSH prior to growth in MM without GSH for 20 h at 37 °C under iron starvation (–Fe), iron sufficiency (+Fe), and shift-iron conditions (sFe). Total RNA was isolated and subject to Northern analysis examining iron-responsive genes. Ribosomal RNA (rRNA) is shown as the control for RNA quality and loading.
