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. 2016 Apr 28;75(6):539–554. doi: 10.1093/jnen/nlw032

FIGURE 2.

FIGURE 2

Neuropathologic assessment of white matter astrogliosis after vascular brain injury (VBI). Neuropathologic scoring of gliosis defined by glial fibrillary acidic protein (GFAP) immunohistochemistry was analyzed in formalin-fixed, paraffin-embedded white matter samples. (A) A No VBI case with minimal white matter astrogliosis. (B) A No VBI case with diffuse white matter astrogliosis. (C) A VBI case with diffuse white matter astrogliosis. (D) Comparison of ordinal gliosis index scores for VBI and No VBI cases demonstrates that this approach did not identify significant differences between the 2 groups (median score: 3 for both groups). (E) GFAP immunolabeling in white matter after histelide preparation in a No VBI case with minimal white matter astrogliosis. (F) A No VBI case with diffuse white matter astrogliosis. (G) A VBI case with diffuse white matter astrogliosis. (H) Graph of the mean absorbance between No VBI and VBI groups determined by histelide. There was no significant difference between the calculated means for the 2 groups (p = 0.79) by Student t -test. Box and whiskers plot shows minimum, 25th percentile, median, 75th percentile, and maximum. Scale bars = 100 µm.