Figure 4.

Recombinant Klotho (rKL) protein administration attenuates lipopolysaccharide (LPS)-mediated endothelial activation. Renal messenger RNA (mRNA) expression levels of E-selectin, vascular cell adhesion protein (VCAM)-1, and intercellular adhesion molecule (ICAM)-1 in control vehicle-treated mice (n = 8) or LPS (1 mg/kg) challenged mice (LPS; n = 8), or LPS (1 mg/kg) challenged mice that received recombinant Klotho (0.05 mg/kg) 30 min prior to LPS injection (rKL + LPS; n = 8). All mice were killed 4 hr after LPS or vehicle injection. Each dot represents an individual mouse. Bars represent the mean ± sd. *p < 0.05, **p < 0.005. GAPDH = glyceraldehyde 3-phosphate dehydrogenase, PBS = phosphate-buffered saline