Figure 5.

Klotho levels are reduced in the brain of endotoxemic mice and are rescued by recombinant Klotho administration prior to lipopolysaccharide (LPS) challenge. Brain messenger RNA (mRNA) expression levels of E-selectin, vascular cell adhesion protein (VCAM)-1, intercellular adhesion molecule (ICAM)-1, neutrophil gelatinase-associated lipocalin (NGAL), interleukin (IL)–6, IL-8, IL-1β, and tumor necrosis factor (TNF)–α in control vehicle-treated mice (n = 5) or LPS (1 mg/kg) challenged mice (LPS; n = 8), or LPS (1 mg/kg) challenged mice that received recombinant Klotho (0.05 mg/kg) 30 min prior to LPS injection (recombinant Klotho [rKL] + LPS; n = 8). All mice were killed 4 hr after LPS or vehicle treatment. All mRNA expression levels were determined by quantitative reverse transcription polymerase chain reaction using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as the housekeeping gene. Each dot represents an individual mouse. Bars represent the mean ± sd. *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001.