Fig. 2.

The formation of astrogliosis on day 14 after spinal cord injury (SCI; scale bar=100 µm). (a) Digital images of astrocytes immunostained for glial fibrillary acidic protein (GFAP; green) and trichrome stained for 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; blue) and anti-F4/80 (red). Astrogliosis is observed in knockout (KO) mice, but the intensity is lower than that in wild-type (WT) mice. (b) Area showing positive immunoreactivity to F4/80 and negative immunoreactivity to GFAP. No differences were observed in the F4/80-positive and GFAP-negative areas between WT and KO mice.