Figure 7. Enhanced action of PdBu at synapses depleted of both Rop and RIM.

(A) Paired-pulse ratio is plotted against initial EPSC amplitude for the indicated genotypes and conditions (in the absence and presence of PdBu). (B) Representative traces for the indicated genotypes in the absence (baseline) and presence of PdBu (PdBu, red). (C) The effect of PdBu application is plotted for wild type controls, the Rop^G27/rim¹⁰³ double heterozygous condition and each heterozygous mutation alone. Each genotype is expressed as a percent change in the presence compared to absence of PdBu. Calculations are made on the first EPSC of the stimulus train (EPSC1). The average percent change is statistically significant only in the double heterozygous condition (p<0.01; ANOVA, One Way with Tukey Multiple Comparisons). (D) Data as in (C) plotting the ratio of EPSC4/EPSC1 as a percent change in the presence compared to absence of PdBu. The average percent change is statistically significant only in the double heterozygous condition (p<0.01; ANOVA, One Way with Tukey Multiple Comparisons).