Table 1. Empirical results for the effect of irx3b morpholino on cdh17, stc1 and slc12a1 expression at 24 hpf, 36 hpf and 50 hpf Un-injected wild-type embryos showed no phenotypes in the pronephros.

The phenotypes for irx3b morphants are: slc12a1, reduction in staining; stc1, increased/ectopic staining; cdh17, expanded DL domain, reduced DE staining and reduced/stretched PST segment. Experiments were repeated multiple times (at least three times) and the results showed are from one representative experiment.

slc12a1/stc1	24 hpf	36 hpf	50 hpf
Un-injected	33	34	33
irx3bmo slc12a1 phenotype	17/23	27/32	30/39
irx3bmo stc1 phenotype	18/23	27/32	33/39

cdh17/stc1	24 hpf	36 hpf	50 hpf
Un-injected	27	30	31
irx3bmo cdh17 phenotype	25/27	29/31	30/38
irx3bmo stc1 phenotype	22/27	26/31	31/38

cdh17/slc12a1	24 hpf	36 hpf	50 hpf
Un-injected	23	23	27
irx3bmo cdh17 phenotype	24/27	29/30	32/35
irx3bmo slc12a1 phenotype	26/27	27/30	34/35