Figure 7. High-resolution refinement: -galactosidase (A) FSC curves between independently refined half-maps for the different stages of processing as explained in the main text.
(B) FSC curves between PDB-5A1A and the maps at the same stages of processing as in A. (C) Representative density features for some of the maps for which FSC curves are shown in A and B.
Figure 7—figure supplement 1. FSC curves between PDB-5A1A and EMD-4116, EMD-7770 and the new map reconstructed in RELION-3.
Figure 7—figure supplement 2. The B-factor plot that was generated automatically by the bfactor_plot.py script.
The script performed nine 3D refinement and post-processing jobs with subsets of increasing sizes for the beta-galactosidase data set. Fitting a straight line through the inverse of the resolution squared of each refinement against the natural logarithm of the number of particles in the corresponding subset yielded an estimated B-factor of 56 Å2.