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Isothermal titration microcalorimetry measurement of NQO1 R139W. NQO1 R139W: 35 injections with 6 μL of 298 μm NQO1 R139W apoprotein solution in 29 μm FAD solution and 300 s spacing (top) at 25 °C. From three independent measurements under the same conditions, the dissociation constant was calculated to KD = 155 ± 27 nm. Data was fitted using the one binding site model.
