Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Steady state kinetic parameters for NQO1 and NQO1 R139W. Kinetic parameters with standard deviations determined for NQO1 and NQO1 R139W, using NADH as electron donor and menadione as electron acceptor.
| NADH | Menadione | ||||||
|---|---|---|---|---|---|---|---|
| kcat,app (s−1) | KM,app (mm) | kcat,app/KM,app (mm−1.s−1) | kcat (s−1) | KM(μm) | kcat/KM(μm−1.s–1) | ||
| NQO1 WT | 1590 ± 52 | 3.22 ± 0.27 | 494 ± 44 | 1830 ± 82 | 11.6 ± 2.2 | 158 ± 31 | |
| NQO1 R139W | 1580 ± 73 | 3.31 ± 0.38 | 478 ± 60 | 1730 ± 100 | 10.6 ± 2.8 | 162 ± 44 | |
