Fig. 3.

FIH complexes with importin β1 via HIF1α for nuclear import. (A) U2OS cells were transfected with control siRNA or importin β1 siRNA for 3 days, followed by treatment with DMSO or DMOG (1 mM) for 3 h. Images show immunofluorescence staining of FIH (red) or HIF1α (red) in U2OS cells after the indicated treatments. DAPI (blue) was used to stain nuclei. (B) U2OS cells were transfected with control siRNA or HIF1α siRNA for 3 days, followed by treatment with DMSO or DMOG (1 mM) for 3 h. Total cell lysates from the U2OS cells were immunoprecipitated with an anti-importin β antibody or control IgG. FIH, HIF1α and importin β levels are indicated. Scale bars: 10 µm.