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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Matrix Biol. 2018 Jul 5;74:101–120. doi: 10.1016/j.matbio.2018.07.004

Figure 7: The requirement for laminin is different between basement membrane de novo assembly and repair.

Figure 7:

A) Expression of dsRNA against LanB1 knocked down LanB1-GFP levels in stage 16– 17 embryos, as determined by GFP fluorescence. B) Quantification of GFP fluorescence in embryos showed dsRNA against LanB1 reduced LanB1-GFP levels by 68%. C) For comparison, expression of the same dsRNA against LanB1 in larvae depleted LanB1-GFP by 71% as measured by western blot (see also Fig. S3). D) Expression of dsRNA against LanB1 was sufficient to disrupt collagen IV deposition into the basement membrane of ventral nerve cord channels in stage 16–17 embryos, as visible in controls (yellow arrows) (N ≥ 3). Scale bar, 50 µm.